Journal:

Article Title: Horizontal Transmission of Candida parapsilosis Candidemia in a Neonatal Intensive Care Unit

doi: 10.1128/JCM.40.7.2363-2369.2002

Figure Lengend Snippet: Electrophoretic separation of chromosome-sized DNA bands of C. parapsilosis strains. Lanes: M, S. cerevisiae used as a marker; 1, 2, 3, 7, and 8, C. parapsilosis isolates recovered, respectively, from the conjunctiva (Cp:c1) and blood (Cp:b1) of the newborn with progeria, from the sink area of the NICU (Cp:e1), and from two health care workers of the NICU (Cp:hcw1 and Cp:hcw2); 4, 5, and 6, genetically unrelated strains of C. parapsilosis isolated, respectively, from an outpatient (Cp:op1), from one health care worker of the NICU (Cp:hcw3), and from the environment of a different ward (Cp:e2); 9 and 10, ATCC 96144 and ATCC 96140, respectively, used as reference strains.

Article Snippet: In order to evaluate whether the karyotype similarities registered for the isolates reflected a close relation among strains endemic in the NICU or were due to a low discriminatory power of PFGE when applied to C. parapsilosis , two unrelated strains, Cp:op1 and Cp:e2 (Fig. , lanes 4 and 6), as well as C. parapsilosis strains ATCC 96144 and ATCC 96140 (Fig. , lanes 9 and 10), were included in the same runs.

Techniques: Marker, Isolation

Journal:

Article Title: Horizontal Transmission of Candida parapsilosis Candidemia in a Neonatal Intensive Care Unit

doi: 10.1128/JCM.40.7.2363-2369.2002

Figure Lengend Snippet: Electrophoretic separation of DNA products obtained by RAPD-PCR from C. parapsilosis strains with RPO2 as a primer. (A) Lanes: M, λ DNA-EcoRI/HindIII used as a marker; 1, 2, 3, 7, and 8, C. parapsilosis isolates recovered, respectively, from the conjunctiva (Cp:c1) and blood (Cp:b1) of the newborn with progeria, from the sink area of the NICU (Cp:e1), and from two health care workers of the NICU (Cp:hcw1 and Cp:hcw2); 4, 5, and 6, genetically unrelated strains of C. parapsilosis isolated, respectively, from an outpatient (Cp:op1), from the environment of a different ward (Cp:e2), and from one health care worker of the NICU (Cp:hcw3); 9 and 10, reference strains ATCC 96140 and ATCC 96144, respectively. Molecular sizes (in kilobases) are shown to the left of the gel. (B) Dendrogram showing the genetic similarity of the strain collection.

Article Snippet: In order to evaluate whether the karyotype similarities registered for the isolates reflected a close relation among strains endemic in the NICU or were due to a low discriminatory power of PFGE when applied to C. parapsilosis , two unrelated strains, Cp:op1 and Cp:e2 (Fig. , lanes 4 and 6), as well as C. parapsilosis strains ATCC 96144 and ATCC 96140 (Fig. , lanes 9 and 10), were included in the same runs.

Techniques: Marker, Isolation